Human Thyroid Stimulating Hormone ELISA Kit (TSH) (ab108660)

Overview

  • Product nameHuman Thyroid Stimulating Hormone ELISA Kit (TSH)
  • Detection methodColorimetric
  • Tests
    1 x 96 test
  • Sample type
    Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.2 U/ml
  • Range
    0.2 µlU/ml - 25 µlU/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Thyroid Stimulating Hormone in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Thyroid Stimulating Hormone in Human serum.

    A 96-well plate has been precoated with anti-Thyroid Stimulating Hormone antibodies. Samples and standards and are added to the wells, where Thyroid Stimulating Hormone in the sample and standards binds to the precoated antibody. After incubation and washing, added Anti-Thyroid Stimulating Hormone HRP conjugate binds to the antibody-Thyroid Stimulating Hormone complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Thyroid Stimulating Hormone in the sample and the intensity is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Human Thyroid Stimulating Hormone ELISA Kit (TSH) images

  • Representative standard curve using ab108660

Protocols

References for Human Thyroid Stimulating Hormone ELISA Kit (TSH) (ab108660)

ab108660 has not yet been referenced specifically in any publications.

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The catalog numbers ab108659 and ab108660 come from the same product line and are easy to compare. The TSH ELISA Kit ab108659 comes with standards in the range of 0-25 uIU/ml, while the TSH ELISA ab108660 is consi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"