Overview

  • Product nameHuman Thyroxine ELISA Kit (Free T4)
    See all Thyroxine (T4) kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    0.5 pg/ml
  • Recovery

    97.8 %

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Thyroxine (T4) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Thyroxine in Human serum and plasma.

     

    A 96-well plate has been precoated with anti-Thyroxine antibodies. Samples and the Thyroxine-HRP conjugate are added to the wells, where Thyroxine in the sample competes with the added
    Thyroxine-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Thyroxine in the sample and the intensity is measured at 450 nm.

     

  • Tested applicationsSuitable for: Competitive ELISAmore details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.

Human Thyroxine ELISA Kit (Free T4) images

  • Representative Standrad Curve using ab108686.

Protocols

References for Human Thyroxine ELISA Kit (Free T4) (ab108686)

ab108686 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab108686.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"