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Abcam’s Thyroxine (T4) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Thyroxine in Human serum and plasma.
A 96-well plate has been precoated with anti-Thyroxine antibodies. Samples and the Thyroxine-HRP conjugate are added to the wells, where Thyroxine in the sample competes with the added
Thyroxine-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Thyroxine in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|1X Thyroxine-HRP Conjugate||1 x 12ml|
|50X Wash Solution||1 x 20ml|
|Anti-Thyroxine IgG Coated Microplate (12 x 8 wells)||1 unit|
|Cover foils||1 unit|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|Thyroxine Standard 0 – 0.0 pg/mL||1 x 1ml|
|Thyroxine Standard 1 – 3.0 pg/mL||1 x 1ml|
|Thyroxine Standard 2 – 9.5 pg/mL||1 x 1ml|
|Thyroxine Standard 3 – 21.0 pg/mL||1 x 1ml|
|Thyroxine Standard 4 – 36.0 pg/mL||1 x 1ml|
|Thyroxine Standard 5 – 70.0 pg/mL||1 x 1ml|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab108686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent dilution.|
ab108686 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"