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ab108661, Thyroxine (T4) Human ELISA Kit is intended for the quantitative determination of Total Thyroxine (T4) concentration in Human serum.
L-Thyroxine (T4) is a hormone that is synthesized and stored in the thyroid gland. Proteolytic cleavage of follicular thyroglobulin releases T4 into the bloodstream. Greater than 99% of Thyroxine (T4) is reversibly bound to three plasma proteins in blood - thyroxine binding globulin (TBG) binds 70%, thyroxine binding pre-albumin (TBPA) binds 20%, and albumin binds 10%. Approximately 0.03% of T4 is in the free, unbound state in blood at any one time.
|Components||1 x 96 tests|
|Enzyme Conjugate Concentrate (11x)||1 x 1.3ml|
|Enzyme Conjugate Diluent||1 x 13ml|
|Sheep anti-Tyroxine (T4) coated microtiter wells||1 x 96 units|
|Stop Solution||1 x 11ml|
|Thyroxine (T4) Reference Standards: 0, 2, 5, 10, 15, and 25 µg/dl||1 pack|
|TMB Substrate Solution||1 x 11ml|
Our Abpromise guarantee covers the use of ab108661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
ab108661 has not yet been referenced specifically in any publications.