Overview

  • Product name
    Human Thyroxine ELISA Kit (T4)
    See all Thyroxine (T4) kits
  • Detection method
    Colorimetric
  • Sample type
    Serum
  • Assay type
    Competitive
  • Sensitivity
    = 0.4 µg/dl
  • Range
    0.4 µg/dl - 25 µg/dl
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab108661, Thyroxine (T4) Human ELISA Kit is intended for the quantitative determination of Total Thyroxine (T4) concentration in Human serum.


    L-Thyroxine (T4) is a hormone that is synthesized and stored in the thyroid gland. Proteolytic cleavage of follicular thyroglobulin releases T4 into the bloodstream. Greater than 99% of Thyroxine (T4) is reversibly bound to three plasma proteins in blood - thyroxine binding globulin (TBG) binds 70%, thyroxine binding pre-albumin (TBPA) binds 20%, and albumin binds 10%. Approximately 0.03% of T4 is in the free, unbound state in blood at any one time.

  • Tested applications
    Suitable for: ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.

Images

  • Thyroxine (T4) Standard Curve

Protocols

References

ab108661 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Thyroid tissue was maintained within a microfluidic culture device, continually perfused with medium. The media effluent coming from the device was tested for the presence of thyroxine as a potential marker of tissue viability/ activity. Although the ELISA was not advertised as validated for cell culture supernatant samples, we endevoured to determine its use in this system. Unfortunately, although the ELISA produced a good standard curve, the results obtained from the samples were not ideal as the medium only controls contained a high threshold of thyroxine - presumably due to bovine thyroxine within the FBS which supplemented the medium. A possible work-around which we are looking into is charcoal stripping the serum before incorporating into the media, in order to remove bovine thyroxine before re-running the ELISA.
Username

Andrew Riley

Verified customer

Submitted Feb 20 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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