Overview

  • Product nameHuman Thyroxine ELISA Kit (T4)
    See all Thyroxine (T4) kits
  • Detection methodColorimetric
  • Sample type
    Serum
  • Assay typeCompetitive
  • Sensitivity
    = 0.4 µg/dl
  • Range
    0.4 µg/dl - 25 µg/dl
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab108661, Thyroxine (T4) Human ELISA Kit is intended for the quantitative determination of Total Thyroxine (T4) concentration in Human serum.


    L-Thyroxine (T4) is a hormone that is synthesized and stored in the thyroid gland. Proteolytic cleavage of follicular thyroglobulin releases T4 into the bloodstream. Greater than 99% of Thyroxine (T4) is reversibly bound to three plasma proteins in blood - thyroxine binding globulin (TBG) binds 70%, thyroxine binding pre-albumin (TBPA) binds 20%, and albumin binds 10%. Approximately 0.03% of T4 is in the free, unbound state in blood at any one time.

  • Tested applicationsSuitable for: ELISAmore details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.

Human Thyroxine ELISA Kit (T4) images

  • Thyroxine (T4) Standard Curve

Protocols

References for Human Thyroxine ELISA Kit (T4) (ab108661)

ab108661 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Thyroid tissue was maintained within a microfluidic culture device, continually perfused with medium. The media effluent coming from the device was tested for the presence of thyroxine as a potential marker of tissue viability/ activity. Although the ELISA was not advertised as validated for cell culture supernatant samples, we endevoured to determine its use in this system. Unfortunately, although the ELISA produced a good standard curve, the results obtained from the samples were not ideal as the medium only controls contained a high threshold of thyroxine - presumably due to bovine thyroxine within the FBS which supplemented the medium. A possible work-around which we are looking into is charcoal stripping the serum before incorporating into the media, in order to remove bovine thyroxine before re-running the ELISA.
Username

Andrew Riley

Verified customer

Submitted Feb 20 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"