Overview

  • Product nameHuman Thyroxine ELISA Kit (T4)
    See all Thyroxine (T4) kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Low 16 3pg/ml 0.03 9.8%
    Normal 16 14pg/ml 0.06 4.5%
    High 16 36pg/ml 0.22 6.2%
    Inter-assay
    Sample n Mean SD CV%
    Low 10 34pg/ml 0.04 11.5%
    Normal 10 13.5pg/ml 0.07 3.7%
    HIgh 10 36.9pg/ml 0.25 4.2%
  • Tests
    1 x 96 well plate
  • Sample type
    Serum
  • Assay typeCompetitive
  • Sensitivity
    0.5 ng/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab108664, Thyroxine (T4) Human ELISA Kit is intended for the quantitative determination of Free Thyroxine (T4) concentration in Human serum.

    Thyroxine (T4) or 3,5,3’,5’-tetraiodothyronine is the most commonly measured thyroid hormone for the diagnosis of thyroid function. Thyroxine (T4) has its primary infuence on protein synthesis and oxygen consumption in virtually all tissues but it is also important for growth, development, and sexual maturation. Thyroxine (T4) is synthesized by the thyroid gland and is secreted into the bloodstream. Here the Thyroxine (T4) becomes bound to serum proteins for transport to the cells. The major transport protein is Thyroxine Binding Globulin (TBG) which normally accounts for 80% of the bound Thyroxine (T4). Other thyroid hormone binding proteins are Thyroxine Binding Prealbumin and Albumin. Most of the serum Thyroxine (T4) is bound to these transport proteins leaving only about 0.03% free to exert its effect on cells. It is the free Thyroxine (T4) (fT4) that represents the metabolically active fraction; for this reason the measurement of fT4 concentration is considered to be an indicator of patient thyroid status. Primary hypothyroidism results in underproduction of Thyroxine (T4) by the thyroid gland and consequently an abnormally low circulating fT4 concentration in the blood. Primary hyperthyroidism leads to excessive thyroid production on Thyroxine (T4) and resulting elevated fT4 concentration.

  • Tested applicationsSuitable for: Competitive ELISAmore details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.

Human Thyroxine ELISA Kit (T4) images

  • Standard Curve

Protocols

References for Human Thyroxine ELISA Kit (T4) (ab108662)

ab108662 has not yet been referenced specifically in any publications.

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