The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-TIA1 antibody (ab40693)
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Cytotoxic granule associated RNA binding protein
Cytotoxic granule associated RNA binding protein 1
Nucleolysin TIA 1 isoform p40
Nucleolysin TIA-1 isoform p40
Nucleolysin TIA1 isoform p40
p40 TIA 1
p40-TIA-1 (containing p15-TIA-1)
RNA binding protein TIA 1
RNA binding protein TIA1
RNA-binding protein TIA-1
T-cell-restricted intracellular antigen-1
TIA 1 cytotoxic granule associated RNA binding protein
TIA1 cytotoxic granule associated RNA binding protein
TIA1 cytotoxic granule associated RNA binding protein like 1
FunctionInvolved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.