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Abcam’s anti-Tick-borne encephalitis (TBE) virus IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Tick-borne encephalitis virus in Human serum and plasma.
A 96-well plate has been precoated with Tick-borne encephalitis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Tick-borne encephalitis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tick-borne encephalitis IgM sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover foil||1 unit|
|IgM Sample Diluent||Green, White cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|Tick-borne encephalitis (IgM) Coated Microplate (12 x 8 wells)||12 strips of 8 wells||1 unit|
|Tick-borne encephalitis virus anti-IgM HRP Conjugate||Red, Black cap||1 x 20ml|
|Tick-borne encephalitis virus IgM Cut-off Control||Yellow, Green cap||1 x 3ml|
|Tick-borne encephalitis virus IgM Negative Control||Yellow, Blue cap||1 x 2ml|
|Tick-borne encephalitis virus IgM Positive Control||Yellow, Red cap||1 x 2ml|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent dilution.|
ab108774 has not yet been referenced specifically in any publications.