Overview

  • Product name
    Human TIE1 ELISA Kit
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 0.6 ng/ml
  • Range
    0.68 ng/ml - 500 ng/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 105.9 93% - 115%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s TIE1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIE1 in cell culture supernatants.


    This assay employs an antibody specific for Human TIE1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIE1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIE1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIE1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Human Tie-1 in normal serum with ab100649, only in serum spiked with Human Tie-1. Plasma is not recommended.

  • Notes

    Serum has been validated as a sample matrix with this kit. However, TIE1 concentrations are low in normal serum and therefore may not be detected by this assay.

    Plasma is not recommended.

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human TIE1 2 vials
    Recombinant Human TIE1 Standard 2 vials
    Stop Solution 1 x 25ml
    TIE1 Microplate (12 x 8 wells) 1 unit
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    TIE1/TIE (tyrosine kinase with Ig and EGF homology domains 1) and TIE2/Tek define a new class of the receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin like domains flanking three epidermal growth factor (EFG) like domains followed by three fibronectin type III like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. Human TIE1 cDNA encodes a 1138 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Due to glycosylation, the protein migrates to approximately 160 kDa in SDS PAGE under reducing conditions. TIE1 and TIE2, expressed primarily on endothelial and hematopoietic progenitor cells, play important roles in angiogenesis, vasculogenesis, and hematopoiesis. In developing vascular endothelial cells, TIE1 and TIE2 are specifically expressed. Two ligands that bind TIE have been identified, angiopoietin 1 and angiopoietin 2. Based on gene targeting studies, the in vivo functions of TIE1 are related to endothelial cell differentiation. The receptor tyrosine kinase TIE also plays a role in the survival and integrity of the endothelium.
  • Alternative names
    • EC 2.7.10.1
    • JTK14
    • receptor tyrosine kinase
    • soluble TIE1 variant 1
    • soluble TIE1 variant 2
    • soluble TIE1 variant 3
    • soluble TIE1 variant 4
    • soluble TIE1 variant 5
    • TIE
    • tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1
    • tyrosine kinase with immunoglobulin like and EGF like domains 1
    • tyrosine-protein kinase receptor Tie 1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100649

  • Representative standard curve using ab100649

Protocols

References

ab100649 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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