Abcam’s TIE2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIE2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human TIE2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIE2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIE2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIE2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
FunctionThis protein is a protein tyrosine-kinase transmembrane receptor for angiopoietin 1. It may constitute the earliest mammalian endothelial cell lineage marker. Probably regulates endothelial cell proliferation, differentiation and guides the proper patterning of endothelial cells during blood vessel formation.
Tissue specificityPredominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.
Involvement in diseaseDefects in TEK are a cause of dominantly inherited venous malformations (VMCM) [MIM:600195]; an error of vascular morphogenesis characterized by dilated, serpiginous channels.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily. Contains 3 EGF-like domains. Contains 3 fibronectin type-III domains. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain.
Post-translational modificationsPhosphorylated. Phosphorylation is induced by ANGPT1 and ANGPT2. ANGPT1-induced phosphorylation is impaired during hypoxia. Dephosphorylated by PTPRB. Autophosphorylation at Tyr-1108 is critical for coupling downstream cell migration signal transduction pathways with ANG1 stimulation in endothelial cells.