Overview

  • Product name
    Human TIMP1 ELISA Kit
    See all TIMP1 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    PBMC SN 5 2.7%
    Inter-assay
    Sample n Mean SD CV%
    PBMC SN 3 5%
  • Sample type
    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    23 pg/ml
  • Range
    78 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 105 104% - 108%
    Cell culture media 103 100% - 108%
    Heparin Plasma 97 94% - 102%
    EDTA Plasma 108 102% - 117%
    Citrate Plasma 98 95% - 101%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat, Rabbit, Guinea pig, Hamster, Dog, Pig
  • Product overview

    ab187394 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TIMP Metalloproteinases Inhibitor 1 (TIMP1) protein in human serum, plasma, and cell culture supernatant samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Tissue inhibitor of metalloproteinases 1 (TIMP1 or Metalloproteinase inhibitor 1) is a widely expressed inhibitor of matrix metalloproteinases (MMPs). It functions by binding non-covalently to MMPs (in a 1:1 stoichiometry) and blocking access of substrate to the MMP active site. TIMP1 expression is induced by pro-inflammatory cytokines. It is a soluble factor found circulating in serum and plasma. TIMP1 appears also have functions that are independent of MMP inhibition, including promoting erythropoiesis and inhibiting apoptosis in B cells.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab187394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • The TIMP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • PBMC were grown in the absence or presence of phytohemagglutinin (PHA) for 2 days. TIMP1 was measured in 2, 4, 8, 16 and 32-fold diluted cell culture supernatants of unstimulated and PHA stimulated PBMC. Raw values (Mean +/-SD, n=2) are graphed.

  • Background subtracted data from duplicate measurements are plotted.

  • Serum from 10 apparently healthy male donors was measured in triplicate. The mean TIMP1 concentration was determined to be 109 ng/mL with a range of 85 – 125 ng/mL.

  • PBMC were grown in the absence (unstimulated) or presence of phytohemagglutinin (PHA) (stimulated) for 2 days (stimulated). TIMP1 was measured in 2-fold diluted cell culture supernatants of unstimulated and PHA stimulated PBMC. Measured values were interpolated from the TIMP1 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 10 ng/mL unstimulated and 8.2 ng/mL stimulated.

Protocols

References

This product has been referenced in:
  • He Z  et al. Cardiomyocyte-specific expression of CYP2J2 prevents development of cardiac remodelling induced by angiotensin II. Cardiovasc Res 105:304-17 (2015). Read more (PubMed: 25618409) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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