Overview

  • Product nameHuman TIMP2 ELISA Kit
    See all TIMP2 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    1.6 pg/ml - 1200 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.53 84% - 103%
    Serum 94.28 83% - 103%
    Plasma 93.69 82% - 102%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human TIMP-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIMP-2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human TIMP-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIMP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. The activation and activity of MMPs are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP2 (also called CSC-21K) is a 24 kDa glycoprotein that is expressed by a variety of cell types. It forms a non covalent, stoichiometric complex with both latent and active MMPs. TIMP2 shows a preference for MMP2. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models. TIMP2 is constitutively produced in low (pg/ml) levels by most cell types in tissue culture. The low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.
  • Cellular localizationSecreted
  • Alternative names
    • CSC 21K
    • CSC21K
    • D11Bwg1104e
    • DDC8
    • Metalloproteinase inhibitor 2
    • Metalloproteinase inhibitor 2 precursor
    • MGC105282
    • OTTMUSP00000004170
    • OTTMUSP00000004171
    • RP23-394O9.2
    • TIMP 2
    • TIMP metallopeptidase inhibitor 2
    • Tissue inhibitor of metalloproteinase 2
    • Tissue inhibitor of metalloproteinases 2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human TIMP2 ELISA Kit images

  • Representative standard curve using ab100653

  • Representative standard curve using ab100653

Protocols

References for Human TIMP2 ELISA Kit (ab100653)

This product has been referenced in:
  • Ruiz-Zapata AM  et al. Functional characteristics of vaginal fibroblastic cells from premenopausal women with pelvic organ prolapse. Mol Hum Reprod 20:1135-43 (2014). Read more (PubMed: 25189765) »

See 1 Publication for this product

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Please note this product is unpublished so when you decide to order you need to contact our customer service team for them to put this item in your order.

I hope this info will help. Please let me know if you experience any difficulties.

This product is available but not published on our website. You cannot purchase this using our normal ordering system however I can add this product to your next shipment. Could you confirm how many vials customer would need..6?.
In my last I made...

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Thank you for contacting us. I apologize for the delay.

We are ready to publish this product. The product would be available at US dollar 120 per 200ul. The customer would need 6 vials of product so it will cost them $720 excluding shipping c...

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Thank you for your email. I am sorry for the delay while I was in contact with lab.

The volume of HRP-Streptavidin is very small and therefore would be difficult to see with the naked eye. The HRP-streptavidin volume sent was 8ul and is very ...

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Thank you very much for contacting. I apologize for the delay as we were conducting some tests.

We have also seen gloopy things in the Assay buffer which first seen as like fungal growth however when we warmed the buffer at room temperature w...

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Thank you for contacting us. I apologize for the inconvenience caused.

I have started working on your inquiry and have asked our lab to provide details? I will get back to you with more updates soon.

Many thanks for having patience...

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