Overview

  • Product nameHuman TIMP3 ELISA Kit (MIG-5)
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    625 pg/ml - 10000 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human TIMP3 (MIG-5) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human TIMP3 (MIG-5) in cell culture supernatants, serum plasma (heparin, EDTA) and saliva.

    A TIMP3 (MIG-5) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for TIMP3 (MIG-5) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human TIMP3 (MIG-5) amount of sample captured in plate.

  • Notes

    Note: In our lab we were only able to detect high doses of TIMP3 above 625 pg/ml.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
  • Involvement in diseaseDefects in TIMP3 are the cause of Sorsby fundus dystrophy (SFD) [MIM:136900]. SFD is a rare autosomal dominant macular disorder with an age of onset in the fourth decade. It is characterized by loss of central vision from subretinal neovascularization and atrophy of the ocular tissues. Generally, macular disciform degeneration develops in the patients eye within 6 months to 6 years.
  • Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Alternative names
    • HSMRK222
    • K222
    • K222TA2
    • Metalloproteinase inhibitor 3
    • MIG 5 protein
    • Protein MIG-5
    • SFD
    • TIMP metallopeptidase inhibitor 3
    • TIMP-3
    • TIMP3
    • TIMP3_HUMAN
    • Tissue inhibitor of metalloproteinases 3
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human TIMP3 ELISA Kit (MIG-5) images

  • TIMP3 measured in culture supernatants (duplicates +/- SD).

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Representative standard curve using ab119608.

Protocols

References for Human TIMP3 ELISA Kit (MIG-5) (ab119608)

This product has been referenced in:
  • Cheng KS  et al. Circulating matrix metalloproteinase-2 and -9 enzyme activities in the children with ventricular septal defect. Int J Biol Sci 9:557-63 (2013). Human . Read more (PubMed: 23847438) »

See 1 Publication for this product

Product Wall

We do not have in-house data for plasma but the reference listed on the datasheet used this TIMP3 ELISA to assay plasma from children. The levels in normal children averaged 1230pg/mL. So, to fall within the range of the standards, the samples were eit...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"