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Abcam’s Human TIMP3 (MIG-5) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human TIMP3 (MIG-5) in cell culture supernatants, serum plasma (heparin, EDTA) and saliva.
A TIMP3 (MIG-5) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for TIMP3 (MIG-5) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human TIMP3 (MIG-5) amount of sample captured in plate.
Note: In our lab we were only able to detect high doses of TIMP3 above 625 pg/ml.
|Components||1 x 96 tests|
|ABC Diluent Buffer||1 x 12ml|
|Antibody Diluent Buffer||1 x 12ml|
|Anti-Human TIMP3 antibody Microplate (12 x 8 wells)||1 unit|
|Avidin-Biotin-Peroxidase Complex (ABC)||1 x 130µl|
|Biotinylated anti-Human TIMP3 antibody||1 x 130µl|
|Lyophilized recombinant Human TIMP3 standard||2 x 10ng|
|Plate Seal||4 units|
|Sample Diluent Buffer||1 x 30ml|
|TMB Color Developing Agent||1 x 10ml|
|TMB Stop Solution||1 x 10ml|
Our Abpromise guarantee covers the use of ab119608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
TIMP3 measured in culture supernatants (duplicates +/- SD).
Standard curve with background signal subtracted (duplicates; +/- SD).
Representative standard curve using ab119608.