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Abcam’s Tissue Factor (CD142) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Factor concentrations in plasma, serum, tissue extracts and cell culture supernatants.
A Tissue Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tissue Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tissue Factor captured in plate.
|Components||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent N Concentrate||1 x 30ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|50X Biotinylated Human Tissue Factor Antibody||1 x 120µl|
|Chromogen Substrate||1 x 8ml|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
|Tissue Factor Microplate (12 x 8 well strips)||1 unit|
|Tissue Factor Standard||1 vial|
Our Abpromise guarantee covers the use of ab108903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
CD142 measured in cell lysates showing quantity (pg) per 1 million cells tested
CD142 measured in various biofluids showing quantity (pg) per mL of tested sample
Representative Standard Curve using ab108903.
ab108903 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"