Human Tissue Factor Pathway Inhibitor ELISA Kit (TFPI) (ab108904)

Overview

  • Product name
    Human Tissue Factor Pathway Inhibitor ELISA Kit (TFPI)
    See all Tissue Factor Pathway Inhibitor kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.4%
  • Sample type
    Cell culture supernatant, Milk, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    > 0.12 ng/ml
  • Range
    0.313 ng/ml - 20 ng/ml
  • Recovery

    96.5 %

  • Assay time
    5h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Tissue Factor Pathway Inhibitor (TFPI) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Factor Pathway Inhibitor concentrations in plasma, serum, milk, cerebrospinal fluid and cell culture samples.


    A Tissue Factor Pathway Inhibitor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tissue Factor Pathway Inhibitor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tissue Factor Pathway Inhibitor captured in plate.


    Get results in 90 minutes with Human Tissue Factor Pathway Inhibitor ELISA Kit (ab188392) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Function
    Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
  • Tissue specificity
    Mostly in endothelial cells.
  • Sequence similarities
    Contains 3 BPTI/Kunitz inhibitor domains.
  • Domain
    This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localization
    Secreted and Microsome membrane.
  • Information by UniProt
  • Alternative names
    • EPI
    • Extrinsic pathway inhibitor
    • LACI
    • Lipoprotein-associated coagulation inhibitor
    • TFPI
    • TFPI1_HUMAN
    • Tissue factor pathway inhibitor
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab108904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Human Tissue Factor Pathway Inhibitor ELISA Kit (TFPI) images

  • TFPI measured in various samples showing quantity (ng) per mL of tested sample

  • Representative Standard Curve using ab108904.

Protocols

References for Human Tissue Factor Pathway Inhibitor ELISA Kit (TFPI) (ab108904)

This product has been referenced in:
  • Park MY  et al. Biomarkers of kidney injury and klotho in patients with atherosclerotic renovascular disease. Clin J Am Soc Nephrol 10:443-51 (2015). Sandwich ELISA ; Human . Read more (PubMed: 25542906) »

See 1 Publication for this product

Product Wall

We can confirm that heparin does not interfere with the assay. However, we are not sure about protamine. This has not been tested before.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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