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|Sample type||Average %||Range|
|Serum||98||77% - 109%|
Abcam’s Tissue type Plasminogen Activator Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Tissue type Plasminogen Activator concentrations in Cell culture supernatant and serum.
Tissue type Plasminogen Activator specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells along with an HRP-conjugated Tissue type Plasminogen Activator detection antibody and the microplate is then incubated at room temperature and unbound proteins are then washed away with a wash buffer. TMB is then added and catalyzed by HRP to produce a blue color product that subsequently changes to yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the Tissue type Plasminogen Activator amount of sample captured on the plate.
For higher sensitivity in only 90 minutes, we recommend Human Tissue Plasminogen Activator ELISA Kit (ab190812) from our SimpleStep ELISA® range.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||2 units|
|HRP-Conjugate anti-human Tissue type Plasminogen Activator polyclonal antibody||1 x 70µl|
|Human Tissue type Plasminogen Activator Standard lyophilized||2 vials|
|Microplate coated with polyclonal antibody to Human Tissue type Plasminogen Activator (12 x 8 wells)||1 unit|
|Sample Diluent||1 x 12ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Representative Standard Curve using ab119563.
ab119563 has not yet been referenced specifically in any publications.