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Abcam’s Tissue Type Plasminogen Activator (TPA) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Type Plasminogen Activator in plasma, serum, urine, saliva, milk, cell culture supernatants and tissue extracts.
A TPA specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TPA specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TPA captured in plate.
Save time with a 90-minute ELISA protocol. Try Human Tissue Plasminogen Activator ELISA Kit (ab190812) from our SimpleStep ELISA® range.
|Components||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent N Concentrate||1 x 30ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|50X Biotinylated Human TPA Antibody||1 x 120µl|
|Chromogen Substrate||1 x 8ml|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
|TPA Microplate (12 x 8 well strips)||1 unit|
|TPA Standard||1 vial|
Our Abpromise guarantee covers the use of ab108914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
Tissue Type Plasminogen Activator measured in biological fluids showing quantity (ng) per mL of tested sample. Samples diluted 2-10 fold.
Tissue Type Plasminogen Activator measured in biological fluids showing quantity (ng) per mL of tested sample. Human samples diluted 3-27 fold. Rat and mouse samples diluted 1-10 fold.
Representative Standard Curve Using ab108914.
ab108914 has not yet been referenced specifically in any publications.