Human TLR2 ELISA Kit (CD282) (ab131556)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (qualitative)
- Reacts with: Human
Overview
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Product name
Human TLR2 ELISA Kit (CD282) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 369pg/ml 18.81 = 4.3% 2 16 2018pg/ml 133.18 = 6.6% 3 16 5303pg/ml 212.12 = 4% Inter-assay Sample n Mean SD CV% 1 24 392pg/ml 22.34 = 5.7% 2 24 1927pg/ml 150.3 = 7.8% 3 24 5643pg/ml 242.64 = 4.3% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (qualitative) -
Sensitivity
< 10 pg/ml -
Range
156 pg/ml - 10000 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human TLR2 (CD282) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human soluble TLR2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
A TLR2 (CD282) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for TLR2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human TLR2 amount of sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human TLR2 coated antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human TLR2 antibody 1 x 100µl Lyophilized recombinant Human TLR2 standard 2 x 10ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Relevance
Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. -
Cellular localization
Membrane; Single-pass type I membrane protein. -
Alternative names
- CD_antigen=CD282
- CD282
- TIL4
see all -
Database links
- Entrez Gene: 7097 Human
- Omim: 603028 Human
- SwissProt: O60603 Human
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab131556 has been referenced in 1 publication.
- Zhang X et al. Involvement of TLR2/4-MyD88-NF-?B signaling pathway in the pathogenesis of intracranial aneurysm. Mol Med Rep 23:N/A (2021). PubMed: 33655339