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Save time with a 90-minute ELISA protocol. Try Human TNF alpha ELISA Kit (ab181421) from our SimpleStep ELISA® range.
Abcam’s TNF alpha Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TNF alpha concentrations in Human cell culture supernatants, serum and plasma.
A TNF alpha specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TNF alpha specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TNF alpha captured in plate. This assay recognizes both natural and recombinant Human TNF alpha.
|Components||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent N Concentrate||1 x 30ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|30X Biotinylated Human TNF alpha Antibody||1 x 180µl|
|Chromogen Substrate||1 x 8ml|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
|TNF alpha Microplate (12 x 8 well strips)||1 unit|
|TNF alpha Standard||1 vial|
Our Abpromise guarantee covers the use of ab108908 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent dilution.|
ab108908 has not yet been referenced specifically in any publications.