Human TNF alpha ELISA Set (without plates) (ab47347)

Overview

  • Product nameHuman TNF alpha ELISA Set (without plates)
    See all TNF alpha kits
  • Tests
    5 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    25 pg/ml - 800 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s TNFα Human in vitro ELISA Set is designed for the quantitative measurement of TNFα in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    The TNF alpha Elipair includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    This Elipair set is intended to measure human TNF alpha in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    Recognizes both natural and recombinant human TNF alpha.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformReagents

Properties

  • RelevanceTNF-alpha is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF-alpha is a potent pyrogen causing fever by direct action or by stimulation of interleukin 1 secretion and is implicated in the induction of cachexia, under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Cellular localizationCell membrane; single-pass type II membrane protein. Processed form: Secreted protein.
  • Alternative names
    • Cachectin
    • TNF
    • TNF alpha
    • TNFA
    • TNFSF2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab47347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
  • Application notesELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Human TNF alpha ELISA Set (without plates) images

    • TNF alpha Standard curve ranging from 25 to 800 pg/mL

    References for Human TNF alpha ELISA Set (without plates) (ab47347)

    ab47347 has not yet been referenced specifically in any publications.

    Product Wall


    The non-biotinylated version of the detection antibody is available on our catalog as ab27381.


    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

    The detection antibody is available as ab27381.

    Thank you for your inquiry.
    HRP-Streptavidin is provided at 1.0mg/ml for a final working concentration of 0.15µg/ml when diluted according to the recommendations.
    I hope this information helps. Please contact us with any other questions.

    Thank you for contacting us.

    We do not sell individual components for ab47347 at the moment.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Us...

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    Thank you very much for your reply.

    I am glad that you have found some kits that will be suitable for your assay. If you need any help standardizing your methods, please let me know and I'll be happy to assist.

    Have a wonderful day.

    Thank you very much for contacting us with your questions, and for your patience while I have been in touch with the lab regarding your enquiry.

    For the TAC kit ab65329, you can use a general lysis buffer such as RIPA to prepare the lysates.T...

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    For ab47347, we provide capture antibody at 1.0mg/ml and detection at 50µg/ml (25µg/vial).
    I hope this is helpful. Please contact me again if you have any further questions.

    Thanks for your enquiry. Please see below for answers to your questions:

    Response to question 1: No, 24 hr drying time is not necessary. After tapping the plate to remove excess liquid from the wells, it should be totally dry much faster than...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"