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|Sample type||Average %||Range|
|Serum||105||87% - 130%|
Abcam’s TNF beta Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human TNF beta concentrations in cell culture supernatant, serum and plasma.
TNF beta specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with HRP-conjugated TNF beta detection antibody and the microplate is then incubated at room temperature and unbound conjugates are then washed away using wash buffer. TMB is the added and catalyzed by HRP to produce a blue color product that subsequently changes to yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the TNF beta amount of sample captured in plate.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||2 units|
|HRP-Conjugate anti-human TNF beta monoclonal antibody||1 x 200µl|
|Microplate coated with monoclonal antibody to Human TNF beta (12 x 8 wells)||1 unit|
|Sample Diluent||1 x 5ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|TMB Substrate Solution||1 x 15ml|
|TNF beta Standard||2 x 50µl|
Our Abpromise guarantee covers the use of ab119561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119561 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"