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Abcam’s anti-Toxocara canis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Toxocara canis in Human serum and plasma.
A 96-well plate has been precoated with Toxocara canis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled Protein A conjugate is added to the wells, which binds to the immobilized Toxocara canis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Toxocara canis IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover foil||1 unit|
|IgG Sample Diluent||Yellow, White cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
|Toxocara canis Coated Microplate (12 x 8 wells)||12 strips of 8 wells||1 unit|
|Toxocara canis Cut-off Control||Yellow, Green cap||1 x 3ml|
|Toxocara canis Negative Control||Yellow, Blue cap||1 x 2ml|
|Toxocara canis Positive Control||Yellow, Red cap||1 x 2ml|
|Toxocara canis Protein A HRP Conjugate||Blue, Black cap||1 x 20ml|
Our Abpromise guarantee covers the use of ab108775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent dilution.|
ab108775 has not yet been referenced specifically in any publications.