Human Anti-Toxocara canis IgG ELISA Kit (ab108775)

Overview

  • Product name
    Human Anti-Toxocara canis IgG ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pos Serum 12 2.6%
    Inter-assay
    Sample n Mean SD CV%
    Pos. Serum 5 8.1%
  • Sample type
    Serum, Plasma
  • Assay type
    Indirect
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Toxocara canis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Toxocara canis in Human serum and plasma.

     

    A 96-well plate has been precoated with Toxocara canis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled Protein A conjugate is added to the wells, which binds to the immobilized Toxocara canis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Toxocara canis IgG sample captured in plate.

  • Tested applications
    Suitable for: Indirect ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent dilution.

Protocols

References

ab108775 has not yet been referenced specifically in any publications.

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