Human Anti-Toxoplasma gondii IgG ELISA Kit (ab108776)

Overview

  • Product name
    Human Anti-Toxoplasma gondii IgG ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Standard B 6 5.7%
    Standard C 6 5.3%
    Standard D 6 6.2%
    Inter-assay
    Sample n Mean SD CV%
    Standard B 4 7.1%
    Standard C 4 10.1%
    Standard D 4 6.3%
  • Sample type
    Serum, Plasma
  • Assay type
    Indirect
  • Sensitivity
    < 1 IU/ml
  • Range
    1 U/ml - 200 U/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Toxoplasma gondii IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Toxoplasma gondii in Human serum and plasma.

     

    A 96-well plate has been precoated with Toxoplasma gondii antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Toxoplasma gondii-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Toxoplasma gondii IgG sample captured in plate.

  • Tested applications
    Suitable for: Indirect ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent dilution.

Images

  • Representative Standard Curve using ab108776

Protocols

References

ab108776 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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In regards to the Toxoplasma gondii IgG ELISA, ab108776, the wells in this kit are coated with tachyzoites.

I also asked about the expected reads for Standard A in comparison with the blank. In this ap...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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