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Abcam’s anti-Toxoplasma gondii IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Toxoplasma gondii in Human serum and plasma.
A 96-well plate has been precoated with Toxoplasma gondii antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Toxoplasma gondii-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Toxoplasma gondii IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover foil||1 unit|
|IgG Sample Diluent||Yellow, White cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
|Toxoplasma gondii (IgG) Coated Microplate (12 x 8 wells)||12 strips of 8 wells||1 unit|
|Toxoplasma gondii anti-IgG HRP Conjugate||Black cap||1 x 20ml|
|Toxoplasma gondii IgG Standard A – 0 U/mL||Yellow, Blue cap||1 x 2ml|
|Toxoplasma gondii IgG Standard B – 50 U/mL||Yellow, Green Cap||1 x 2ml|
|Toxoplasma gondii IgG Standard C – 100 U/mL||Yellow, Yellow cap||1 x 2ml|
|Toxoplasma gondii IgG Standard D – 200 U/mL||Yellow, Red Cap||1 x 2ml|
Our Abpromise guarantee covers the use of ab108776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent dilution.|
ab108776 has not yet been referenced specifically in any publications.