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ab108778 Toxoplasma gondii IgM Human ELISA Kit is intended for the qualitative determination of IgM class antibodies against Toxoplasma gondii in human serum or plasma (citrate).
Toxoplasma gondii is a small intracellular parasite, whose live cycle has a sexual and an asexual phase. Sexual development is restricted to the intestinal cells of (probably exclusively) cats; the oocysts formed are excreted and due to their resistant cell walls they may be infectious under advantageous circumstances for at least 1 year. Animals and man are intermediate hosts for the asexual proliferation of T. gondii: the ingested parasites will proliferate explosively within the host cells lysing them eventually. They disseminate throughout the body via circulation and lymphatic system and may infect any cell type. In muscle and brain cells cysts are formed which are spheroidal and about 5-100 µm in diameter. Cysts are virtually immortal in the intermediate host.
Toxoplasma gondii is the most common parasite in humans, but its abundance (7-80%) is highly dependent on the geographic area, the socio-economic status and the nutritional customs. Infection only rarely causes toxoplasmosis and usually clinical symptoms are absent, but may produce severe problems in immunosuppressed persons and fetus.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Anti-Human (IgM) Coated Microplate (12 x 8 wells)||12 strips of 8 wells||1 unit|
|Cover foil||1 unit|
|IgM Sample Diluent||Colored yellow||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
|Toxoplasma gondii HRP Conjugate||1 x 15ml|
|Toxoplasma gondii IgM Cut-off Control||Colored yellow; Green cap||1 x 3ml|
|Toxoplasma gondii IgM Negative Control||Colored yellow; Blue cap||1 x 2ml|
|Toxoplasma gondii IgM Positive Control||Colored yellow; Red cap||1 x 2ml|
Our Abpromise guarantee covers the use of ab108778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab108778 has not yet been referenced specifically in any publications.