The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-TRA2B antibody (ab31353)
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Arginine/serine-rich splicing factor 10
Splicing factor arginine/serine rich 10
Transformer 2 beta homolog
Transformer-2 protein homolog B
Transformer-2 protein homolog beta
FunctionSequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.
Tissue specificityHighest expression in heart, skeletal muscle and pancreas. Less abundant in kidney, placenta and brain. Lowest expression in kidney and liver.
Sequence similaritiesBelongs to the splicing factor SR family. Contains 1 RRM (RNA recognition motif) domain.
Post-translational modificationsPhosphorylated in the RS domains. Dimethylation at Arg-241 is probably asymmetric.