Overview

  • Product nameHuman TRAIL ELISA Kit (CD253)
    See all TRAIL kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 40 1500pg/ml 82.9 5.5%
    B 33 116.3pg/ml 8.7 7.4%
    Inter-assay
    Sample n Mean SD CV%
    A 12 1619pg/ml 62.8 3.8%
    B 12 162.8pg/ml 13.1 8%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 64 pg/ml
  • Range
    93.75 pg/ml - 3000 pg/ml
  • Assay time
    3h 45m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s TRAIL (CD253) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TRAIL in cell culture supernatants, buffered solutions or Human serum, plasma, and other body fluids.

    A monoclonal antibody specific for TRAIL has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TRAIL concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TRAIL are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TRAIL present in the samples.

    This kit will recognize both endogenous and recombinant Human TRAIL.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceTRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of TRAIL may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.
  • Cellular localizationCell Membrane; single-pass type II membrane protein.
  • Alternative names
    • Apo 2 ligand
    • APO2L
    • CD253
    • Protein TRAIL
    • TL2
    • TNF related apoptosis inducing ligand
    • Tumor necrosis factor ligand superfamily member 10
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human TRAIL ELISA Kit (CD253) images

  • TRAIL measured in undiluted cell culture supernatants from control (-) or stimulated ((P/I); 48 hours with 50 ng x mL-1 PMA (ab120297) and 1 uM ionomycin (ab120116)) Jurkat cells (duplicates +/- SD).

  • TRAIL measured in undiluted biological fluids (duplicates +/- SD).

  • Representative Standard Curve using ab46074

Protocols

References for Human TRAIL ELISA Kit (CD253) (ab46074)

This product has been referenced in:
  • Greenwood EJ  et al. Simian Immunodeficiency Virus Infection of Chimpanzees (Pan troglodytes) Shares Features of Both Pathogenic and Non-pathogenic Lentiviral Infections. PLoS Pathog 11:e1005146 (2015). Sandwich ELISA . Read more (PubMed: 26360709) »

See 1 Publication for this product

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Thank you for your call today and for letting us know about the trouble with ab46074.
As we discussed, I'm sending a free of charge replacement kit, which should arrive tomorrow on the order ***.
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At this time we haveno information regarding the cross reactivity between our human TRAIL ELISA (ab46074)and the murine protein.

Therefore, I can offer a discount off a future purchase if you buy ab46074 now, test it inmouse and submit feedba...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"