Overview

  • Product name
    Human Transferrin ELISA Kit
    See all Transferrin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Sample type
    Serum, Plasma
  • Assay type
    Competitive
  • Sensitivity
    = 0.09 µg/ml
  • Range
    0.098 µg/ml - 25 µg/ml
  • Recovery

    99 %

  • Assay time
    2h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Transferrin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Transferrin in plasma and serum samples.

    A Transferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Transferrin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Transferrin captured in plate.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 1 x 30ml
    6X Biotinylated Human Transferrin (Lyophilized) 1 vial
    Chromogen Substrate 1 x 8ml
    Human Transferrin Standard 1 x 75µg
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
    Transferrin Microplate (12 x 8 well strips) 1 unit
  • Research areas
  • Function
    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • Tissue specificity
    Expressed by the liver and secreted in plasma.
  • Involvement in disease
    Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • Sequence similarities
    Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Apotransferrin
    • Beta 1 metal binding globulin
    • Beta-1 metal-binding globulin
    • DKFZp781D0156
    • PRO1400
    • PRO1557
    • PRO2086
    • Serotransferrin
    • Serotransferrin precursor
    • Siderophilin
    • TF
    • TFQTL1
    • Transferin
    • Transferrin
    • TRFE_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab108911.

Protocols

References

This product has been referenced in:
  • Hu Y  et al. Investigation on Abnormal Iron Metabolism and Related Inflammation in Parkinson Disease Patients with Probable RBD. PLoS One 10:e0138997 (2015). ELISA ; Human . Read more (PubMed: 26431210) »
  • Gomes JA  et al. Brain iron metabolism and brain injury following subarachnoid hemorrhage: iCeFISH-pilot (CSF iron in SAH). Neurocrit Care 21:285-93 (2014). ELISA ; Human . Read more (PubMed: 24710655) »

See all 3 Publications for this product

Customer reviews and Q&As



The transferring kit contains two antibodies against full length recombinant protein and purified proteins. Human IgG3 kit contains two antibodies against full length purified proteins.

I am afraid we do not map the epitopes of thes...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up