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|Sample type||Average %||Range|
|Serum||103||101% - 106%|
|Cell culture media||88||87% - 89%|
|Heparin Plasma||104||101% - 107%|
|EDTA Plasma||103||100% - 104%|
|Citrate Plasma||101||100% - 102%|
Transferrin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Transferrin protein in human serum, plasma and cell culture supernatant samples.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
|Components||1 x 96 tests|
|10X Transferrin Capture Antibody||1 x 600µl|
|10X Transferrin Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|Antibody Diluent 4BI||1 x 6ml|
|Plate Seals||1 unit|
|Sample Diluent NS (ab193972)||1 x 50ml|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
|Transferrin Human Lyophilized Purified Protein||2 vials|
Our Abpromise guarantee covers the use of ab187391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Example of Transferrin standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Titration of Human serum within the working range of the assay. Background-subtracted data values (mean +/- SD, n = 3) are graphed.
FObserved Transferrin levels in pooled donor normal Human plasma. Mean Human Transferrin values fall within expected normal reference ranges (WHO).
Observed Transferrin levels in individual donor normal Human serum (n=10). Mean Human Transferrin is 1.05 mg/mL and values fall within expected normal reference ranges (WHO).