Human Transthyretin Multiplex Immunoassay Kit (ab223834)

Overview

  • Product name
    Human Transthyretin Multiplex Immunoassay Kit
    See all Prealbumin kits
  • Detection method
    Flow cytometry-fluorescent
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    HepG2 CM 36 3.97%
    Inter-assay
    Sample n Mean SD CV%
    HepG2 CM 3 2.15%
  • Sensitivity
    0.97 pg/ml
  • Range
    1.52 pg/ml - 3333.33 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture media 96 93% - 97%

  • Species reactivity
    Reacts with: Human
  • Product overview

    Quantify up to 75 proteins and peptides per well, using just 12.5 µL of sample, with FirePlex® immunoassays. The assay takes approximately 3.5 hours, followed by particle analysis using a compatible flow cytometer and data analysis using our free FirePlex Analysis Workbench software.


    Benefits of the assay


    - Quantify both low and high abundance analytes with a typical sensitivity of 0.5 pg /mL and a fully extended dynamic range spanning 4.5 logs


    - Choose from hundreds of analytes to easily build your own custom panel, use a convenient pre-mixed pre-designed panel, or add new analytes to a pre-designed panel


    - Consistent batch-to-batch performance with assays built using the same recombinant monoclonal antibody pairs as our SimpleStep ELISA® and Matched Antibody Pair Kits


    - One FirePlex particle platform for both multiplex miRNA assays and multiplex immunoassays


    Find out more about multiplex immunoassays using FirePlex particles here


    Important - Use of the FirePlex Multiplex Immunoassay Kit also requires the purchase of:


    - a) Human Multiplex Immunoassay Core Kit, ab208203; and


    - b) Multiplex Immunoassay Protein Standard Mix, such as ab223804.


    FirePlex® is a registered trademark in the United States and is an unregistered trademark elsewhere.

  • Notes

    The following information provides the sensitivity range and recommended starting dilutions for validated biological samples.

    Sensitivity range – 0.83 – 1.11 pg/mL

    Recommended Dilution Range

    Cell Culture Supernatant

    Serum

    Plasma-EDTA

    Plasma-Heparin

    Plasma-Citrate

    Urine

    1:4

    1:1 million

    1:1 million

    1:1 million

    1:1 million

    1:4

  • Platform
    Flow

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    75X Human Transthyretin Capture Particles Particle Code 36 1 unit
    Human Transthyretin Biotin Detector 1 unit
  • Research areas
  • Function
    Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
  • Tissue specificity
    Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
  • Involvement in disease
    Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
    Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
    Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis.
  • Sequence similarities
    Belongs to the transthyretin family.
  • Domain
    Each monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.
  • Cellular localization
    Secreted. Cytoplasm.
  • Information by UniProt
  • Alternative names
    • Amyloid polyneuropathy
    • Amyloidosis I
    • ATTR
    • Carpal tunnel syndrome 1
    • CTS
    • CTS1
    • Dysprealbuminemic euthyroidal hyperthyroxinemia
    • Dystransthyretinemic hyperthyroxinemia
    • Epididymis luminal protein 111
    • HEL111
    • HsT2651
    • PALB
    • Prealbumin
    • Prealbumin amyloidosis type I
    • Prealbumin Thyroxine-binding
    • Senile systemic amyloidosis
    • TBPA
    • Thyroxine binding prealbumin
    • Transthyretin
    • TTHY_HUMAN
    • TTR
    • TTR protein
    see all
  • Database links

Images

  • Mean background-subtracted data values are graphed (+/- SD).

  • The concentrations of Transthyretin were interpolated from the Transthyretin standard curves and corrected for sample dilution. 1X diluted samples are as follows: serum 1:2 million, plasma (EDTA) 1:1 million, plasma (heparin) 1:1 million, and plasma (citrate) 1:1 million. The interpolated dilution factor corrected mean values are plotted. The mean Transthyretin concentration was determined to be 213 µg/mL in serum, 416 µg/mL in plasma (EDTA), 395 µg/mL in plasma (Heparin), and 427 µg/mL in plasma (citrate).

  • The concentrations of Transthyretin were interpolated from the Transthyretin standard curve and corrected for sample dilution. 1X diluted samples are as follows: HepG2 supernatant 10%. The interpolated dilution factor corrected mean values are plotted. The mean Transthyretin concentration was determined to be 23.6 ng/mL in HepG2 supernatant.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transthyretin concentration was determined to be 255 µg/mL with a range of 91.2 – 371 µg/mL.

  • The concentrations of Transthyretin were interpolated from the Transthyretin standard curves and corrected for sample dilution. 1X diluted samples are as follows: urine 25%. The interpolated dilution factor corrected values are plotted. The mean Transthyretin concentration was determined to be 1583 pg/mL in urine.

References

ab223834 has not yet been referenced specifically in any publications.

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