Human Anti-Treponema pallidum IgG ELISA Kit (ab108779)

Overview

  • Product nameHuman Anti-Treponema pallidum IgG ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pos. Serum 8 1.5%
    Inter-assay
    Sample n Mean SD CV%
    Pos. Serum 4 5.1%
  • Tests
    1 x 96 test
  • Sample type
    Serum, Plasma
  • Assay typeIndirect
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Treponema pallidum IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Treponema pallidum in Human serum and plasma.

     

    A 96-well plate has been precoated with Treponema pallidum antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Treponema pallidum-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Treponema pallidum IgG sample captured in plate.

  • Tested applicationsSuitable for: Indirect ELISAmore details
  • PlatformMicroplate

Properties

    Applications

    Our Abpromise guarantee covers the use of ab108779 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Indirect ELISA Use at an assay dependent dilution.

    Protocols

    References for Human Anti-Treponema pallidum IgG ELISA Kit (ab108779)

    ab108779 has not yet been referenced specifically in any publications.

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