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Abcam’s Triiodothyronine (T3) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Triiodothyronine (T3) in Human serum.
A 96-well plate has been pre-coated with Anti-Triiodothyronine antibodies. Samples, standards and the Triiodothyronine HRP Conjugate are added to the wells, where Triiodothyronine in the sample competes with the added Triiodothyronine-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Triiodothyronine in the sample and the intensity is measured at 450 nm.
|Components||Identifier||1 x 96 tests|
|Anti-Triiodothyronine Coated Microplate (12 x 8 wells)||96 well plate||1 unit|
|Color Reagent A||1 x 13ml|
|Color Reagent B||1 x 13ml|
|Stop solution||1 x 10ml|
|Triiodothyronine HRP Conjugate||1 x 10.5ml|
|Triiodothyronine Standard 0||1 x 1ml|
|Triiodothyronine Standard 1||1 x 1ml|
|Triiodothyronine Standard 2||1 x 1ml|
|Triiodothyronine Standard 3||1 x 1ml|
|Triiodothyronine Standard 4||1 x 1ml|
|Triiodothyronine Standard 5||1 x 1ml|
Our Abpromise guarantee covers the use of ab108663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent dilution.|
ab108663 has not yet been referenced specifically in any publications.