Overview

  • Product nameHuman Triiodothyronine ELISA Kit (T3)
    See all Triiodothyronine (T3) kits
  • Detection methodColorimetric
  • Tests
    1 x 96 test
  • Sample type
    Serum
  • Assay typeCompetitive
  • Sensitivity
    = 0.2 ng/ml
  • Range
    0.2 ng/ml - 10 ng/ml
  • Recovery

    106 %

    Sample specific recovery
    Sample type Average % Range
    Serum 106 0.68ng/ml - 5.81ng/ml

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Triiodothyronine (T3) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Triiodothyronine (T3) in Human serum.

    A 96-well plate has been pre-coated with goat anti-mouse IgG. Samples, standards and the mouse anti-Triiodothyronine Reagent and Triiodothyronine-HRP conjugate are added to the wells, where Triiodothyronine in the sample competes with the added Triiodothyronine-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol E2 in the sample and the intensity is measured at 450 nm.

  • Tested applicationsSuitable for: Competitive ELISAmore details
  • PlatformMicroplate

Properties

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab108664 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Competitive ELISA Use at an assay dependent dilution.

    Human Triiodothyronine ELISA Kit (T3) images

    • Representative standard curve using ab108664

    Protocols

    References for Human Triiodothyronine ELISA Kit (T3) (ab108664)

    ab108664 has not yet been referenced specifically in any publications.

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