|Sample type||Average %||Range|
|Serum||106||0.68ng/ml - 5.81ng/ml|
Abcam’s Triiodothyronine (T3) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Triiodothyronine (T3) in Human serum.
A 96-well plate has been pre-coated with goat anti-mouse IgG. Samples, standards and the mouse anti-Triiodothyronine Reagent and Triiodothyronine-HRP conjugate are added to the wells, where Triiodothyronine in the sample competes with the added Triiodothyronine-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol E2 in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|10X Triiodothyronine HRP Conjugate||1 x 1.3ml|
|Goat anti-mouse IgG Coated Microplate (12 x 8 wells)||1 x 96 tests|
|HRP-Conjugate Diluent||1 x 13ml|
|Stop Solution||1 x 11ml|
|T3 Antibody Reagent||1 x 7ml|
|T3 Standard 0 - 0 ng/mL||1 x 1ml|
|T3 Standard 1 - 0.75 ng/mL||1 x 1ml|
|T3 Standard 2 - 1.5 ng/mL||1 x 1ml|
|T3 Standard 3 - 3 ng/mL||1 x 1ml|
|T3 Standard 4 - 6 ng/mL||1 x 1ml|
|T3 Standard 5 - 10 ng/mL||1 x 1ml|
|TMB Substrate Solution||1 x 11ml|
Our Abpromise guarantee covers the use of ab108664 in the following tested applications.
|Competitive ELISA||Use at an assay dependent dilution.|
ab108664 has not yet been referenced specifically in any publications.