Overview

  • Product nameHuman TSP2 ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Platelets, Other biological fluids, Tissue Extracts
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    156 pg/ml - 10000 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human TSP2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human TSP2 in cell culture supernatants, serum, plasma (heparin, EDTA) and Human milk.

    A TSP2 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for TSP2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human TSP2 amount of sample captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionAdhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties.
  • Tissue specificityHigh expression in invertebral disk tissue.
  • Involvement in diseaseGenetic variations in THBS2 may be a cause of susceptibility to intervertebral disc disease (IDD) [MIM:603932]; also known as lumbar disk herniation (LDH). IDD is one of the most common musculo-skeletal disorders and the predominant cause of low-back pain and unilateral leg pain.
  • Sequence similaritiesBelongs to the thrombospondin family.
    Contains 3 EGF-like domains.
    Contains 1 TSP C-terminal (TSPC) domain.
    Contains 1 TSP N-terminal (TSPN) domain.
    Contains 3 TSP type-1 domains.
    Contains 8 TSP type-3 repeats.
    Contains 1 VWFC domain.
  • Information by UniProt
  • Alternative names
    • THBS2
    • Thrombospondin-2
    • TSP2_HUMAN
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human TSP2 ELISA Kit images

  • Representative Standard Curve using ab119575.

Protocols

References for Human TSP2 ELISA Kit (ab119575)

ab119575 has not yet been referenced specifically in any publications.

Product Wall


I have contacted the lab and unfortunately, we do not have any evidence that it our TSP2 kit cross reacts with other cytokines in the TSP family, nor does it react with other species.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"