Overview

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • AccessionP61081
    • SpeciesHuman
    • SequenceMGSSHHHHHH SSGLVPRGSH MGSHMIKLFS LKQQKKEEES AGGTKGSSKK ASAAQLRIQK DINELNLPKT CDISFSDPDD LLNFKLVICP DEGFYKSGKF VFSFKVGQGY PHDPPKVKCE TMVYHPNIDL EGNVCLNILR EDWKPVLTIN SIIYGLQYLF LEPNPEDPLN KEAAEVLQNN RRLFEQNVQR SMRGGYIGST YFERCLK
    • Molecular weight24 kDa including tags
    • Amino acids1 to 183
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab128447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity>95% by SDS-PAGE .
    ab128447 was purified using conventional chromatography.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 1.17% Sodium chloride

General info

  • Alternative names
    • hUbc12
    • NEDD8 carrier protein
    • NEDD8 conjugating enzyme Ubc12
    • NEDD8 protein ligase
    • NEDD8-conjugating enzyme Ubc12
    • UBC-RS2
    • UBC12
    • UBC12_HUMAN
    • ube2m
    • ubiquitin carrier protein M
    • Ubiquitin conjugating enzyme E2 M
    • Ubiquitin-conjugating enzyme E2 M
    • ubiquitin-protein ligase M
    • yeast UBC12 homolog
    see all
  • FunctionAccepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
  • PathwayProtein modification; protein neddylation.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
  • DomainBoth the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
  • Target information above from: UniProt accession P61081 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Human UBE2M full length protein images

  • 15% SDS-PAGE analysis of ab128447 (3 µg).

References for Human UBE2M full length protein (ab128447)

ab128447 has not yet been referenced specifically in any publications.

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