Human uPA Receptor/U-PAR ELISA Kit (URKR) (ab119612)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human uPA Receptor/U-PAR ELISA Kit (URKR)
See all uPA Receptor/U-PAR kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
< 4 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human uPA Receptor/U-PAR (URKR) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human uPA Receptor/U-PAR (URKR) in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
A uPA Receptor/U-PAR (URKR) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for uPA Receptor/U-PAR(URKR) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human uPA Receptor amount of sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human uPA Receptor antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human uPA Receptor antibody 1 x 100µl Lyophilized recombinant Human uPA Receptor standard 2 x 10ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. -
Tissue specificity
Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain. -
Sequence similarities
Contains 3 UPAR/Ly6 domains. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- CD 87
- CD87
- CD87 antigen
see all -
Database links
- Entrez Gene: 5329 Human
- Omim: 173391 Human
- SwissProt: Q03405 Human
- Unigene: 466871 Human
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab119612 has been referenced in 2 publications.
- Nantajit D et al. Prognostic significance of uPA and uPAR expression in patients with cervical cancer undergoing radiotherapy. Oncol Lett 21:423 (2021). PubMed: 33850564
- Hamie L et al. SuPAR, a potential inflammatory mediator in psoriasis pathogenesis. Clin Exp Pharmacol Physiol 47:1705-1712 (2020). PubMed: 32558953