Human Urokinase type Plasminogen Activator ELISA Kit (ab108917)

Overview

  • Product nameHuman Urokinase type Plasminogen Activator ELISA Kit
    See all Urokinase type Plasminogen Activator kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.2%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Tissue
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.2 ng/ml
  • Range
    0.25 ng/ml - 8 ng/ml
  • Recovery

    98.5 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Urokinase type Plasminogen Activator Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Urokinase type Plasminogen Activator (uPA) in plasma, serum, urine, milk, cell culture supernatants and tissue extracts.

     

    A Urokinase type Plasminogen Activator specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Urokinase type Plasminogen Activator specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Urokinase type Plasminogen Activator captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionSpecifically cleave the zymogen plasminogen to form the active enzyme plasmin.
  • Tissue specificityExpressed in the prostate gland and prostate cancers.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • ATF
    • BDPLT5
    • Plasminogen activator urinary
    • PLAU
    • QPD
    • u PA
    • U-plasminogen activator
    • uPA
    • URK
    • UROK_HUMAN
    • Urokinase-type plasminogen activator chain B
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Human Urokinase type Plasminogen Activator ELISA Kit images

  • Representative Standard Curve using ab108917.

Protocols

References for Human Urokinase type Plasminogen Activator ELISA Kit (ab108917)

ab108917 has not yet been referenced specifically in any publications.

Product Wall



Both ab108914 and ab108917 contain 12, 8 well strips.


You are correct that you should be able to detect tPA and uPA from cell culture supernatants or cell homogenates with both these kits.

Thank you for your email.

We generally recommend our customer using 400-500ul of lysis buffer per 5-10 mg of tissues however you may have to adjust it based upon your results.

500ul lysis buffer for 1 million cells would be ideal.
Read More

Thank you for your email.

We generally recommend our customer using 400-500ul of lysis buffer per 5-10 mg of tissues however you may have to adjust it based upon your results.

500ul lysis buffer for 1 million cells would be ideal.
Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"