Overview

Description

  • Nature
    Synthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab66555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Purity
    70 - 90% by HPLC.

  • Form
    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • 41 kDa ubiquitin specific protease
    • 41 kDa ubiquitin-specific protease
    • Deubiquitinating enzyme 2
    • Ubiquitin carboxyl terminal hydrolase 2
    • Ubiquitin carboxyl-terminal hydrolase 2
    • ubiquitin specific peptidase 2
    • Ubiquitin specific processing protease 2
    • Ubiquitin specific protease 12
    • Ubiquitin specific protease 9
    • Ubiquitin specific protease, 41kD
    • Ubiquitin thioesterase 2
    • Ubiquitin thiolesterase 2
    • Ubiquitin-specific-processing protease 2
    • Ubiquitin-specific-processing protease testis
    • UBP-t
    • UBP2_HUMAN
    • UBP41
    • USP2
    • USP9
    see all
  • Function
    Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Isoform 1 and isoform 4 possess both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity. Has no deubiquitinase activity against p53/TP53. Prevents MDM2-mediated degradation of MDM4. Plays a role in the G1/S cell-cycle progression in normal and cancer cells. Plays a role in the regulation of myogenic differentiation of embryonic muscle cells.
  • Tissue specificity
    Expressed in mesangial cells of the kidney and in different types of glomerulonephritides (at protein level).
  • Sequence similarities
    Belongs to the peptidase C19 family. USP2 subfamily.
  • Domain
    The different N-terminus extensions of isoform 1 and isoform 4 determine their respective subcellular localization and differentiel effect on myoblast fusion and accumulation of muscle-specific proteins. The different N-terminus extensions of isoform 1 and isoform 4 are not essential for their catalytic activity.
  • Cellular localization
    Nucleus and Cytoplasm. Cytoplasm > perinuclear region. Localizes in the spermatid head in late-elongating spermatids in the thin area between the outer acrosomal membrane and the plasma membrane.
  • Information by UniProt

References

ab66555 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab66555.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up