Human uterus tissue lysate - soluble fraction (female, 48 years) (ab44033)

Overview

  • Product nameHuman uterus tissue lysate - soluble fraction (female, 48 years)
    See all Uterus lysates
  • Description
    Human uterus soluble fraction lysate
  • General notes

    Diagnosis: Normal.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44033: Extraction 1: soluble protein fraction ab44034 Human Uterus Tumor Tissue lysate ab44033 Human Uterus Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44036 Human Uterus Tumor Tissue lysate ab44035 Human Uterus Normal Tissue lysate (matched).

Properties

  • FormLiquid
  • Storage instructionsShipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Constituents: PBS, 0.0001% Pepstatin A, 0.018% Sodium orthovanadate, 0.004% Sodium fluoride, 0.0001% Aprotinin, 0.0001% Leupeptin, 0.25% Sodium deoxycholate, 0.017% PMSF, 0.1% SDS, 0.029% EDTA
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Research areas

    References for Human uterus tissue lysate - soluble fraction (female, 48 years) (ab44033)

    ab44033 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. For determining the concentrations, the Bio-Rad Protein Assay reagent is used- based on the Bradford method for determining protein concentration. BSA and IgG are used to plot the standard curve used to determine tissue ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"