Human uterus tumor tissue lysate (adenosquamous cell carcinoma) - insoluble fraction (ab44079)

Overview

  • Product nameHuman uterus tumor tissue lysate (adenosquamous cell carcinoma) - insoluble fraction
    See all Uterus lysates
  • Description
    Hu uterus insoluble fraction lysate
  • General notesTissue: Uterus Location: Uterus Diagnosis: Adenosquamous cell carcinoma Stage: Not recorded Grade: Not recorded Sex: Female Age: 54 years Appearance: Macroscopic - Tumor 7 cm in diameter, located at the bottom of the uterus, has flower shape. Cut section is soft and pink. Microscopic - Tumor is composed of epithelial cells having basophilic, irregular nuclei and abundant cytoplasm. Nuclear chromatin is coarse and nucleoli are prominent. Mitoses are evident. The tumor cells form clusters. The stroma shows many congestive blood vessels and inflammatory cells including lymphocytes and histocytes. Insoluble protein fraction


    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44079: Extraction 1: soluble protein fraction ab44077 Human Uterus Tumor Tissue lysate ab44076 Human Uterus Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44079 Human Uterus Tumor Tissue lysate ab44078 Human Uterus Normal Tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.

    References for Human uterus tumor tissue lysate (adenosquamous cell carcinoma) - insoluble fraction (ab44079)

    ab44079 has not yet been referenced specifically in any publications.

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