Overview

  • Product name
    Human VAP-1 ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8 3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8 4.5%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    19 pg/ml
  • Range
    31.3 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 96 82% - 117%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s VAP-1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VAP-1 in cell culture supernatant, serum, and plasma (EDTA, heparin) and amniotic fluid.

    VAP-1 specific antibodies have been precoated onto 96-well plates. Standards and samples are added to the wells and VAP-1 present in a sample is bound to the wells by the immobilized antibody. After incubation the wells are washed and biotinylated anti-Human VAP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is added to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VAP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab119564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab119564.

Protocols

References

ab119564 has not yet been referenced specifically in any publications.

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