Overview

  • Product nameHuman VAP-1 ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8 3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8 4.5%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    19 pg/ml
  • Range
    31.3 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 96 82% - 117%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s VAP-1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VAP-1 in cell culture supernatant, serum, and plasma (EDTA, heparin) and amniotic fluid.

    VAP-1 specific antibodies have been precoated onto 96-well plates. Standards and samples are added to the wells and VAP-1 present in a sample is bound to the wells by the immobilized antibody. After incubation the wells are washed and biotinylated anti-Human VAP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is added to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VAP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis.
  • Tissue specificityStrongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Also highly expressed in appendix, lung and small intestine. Expressed also in adipose tissue, in bone marrow, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, spleen and testis.
  • Sequence similaritiesBelongs to the copper/topaquinone oxidase family.
  • Post-translational
    modifications
    Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.
    N- and O-glycosylated.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Alternative names
    • Amine oxidase copper containing 3
    • AOC3
    • AOC3_HUMAN
    • Copper amine oxidase
    • HPAO
    • Membrane primary amine oxidase
    • Semicarbazide-sensitive amine oxidase
    • SSAO
    • VAP-1
    • VAP1
    • Vascular adhesion protein 1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human VAP-1 ELISA Kit images

  • Representative Standard Curve using ab119564.

Protocols

References for Human VAP-1 ELISA Kit (ab119564)

ab119564 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab119564.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"