|Sample type||Average %||Range|
|Serum||96||82% - 117%|
Abcam’s VAP-1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VAP-1 in cell culture supernatant, serum, and plasma (EDTA, heparin) and amniotic fluid.
VAP-1 specific antibodies have been precoated onto 96-well plates. Standards and samples are added to the wells and VAP-1 present in a sample is bound to the wells by the immobilized antibody. After incubation the wells are washed and biotinylated anti-Human VAP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is added to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VAP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||2 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||4 units|
|Biotin-Conjugate anti-human VAP-1 monoclonal antibody||1 x 70µl|
|Microplate coated with monoclonal antibody to Human VAP-1 (12 x 8 wells)||1 unit|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 150µl|
|TMB Substrate Solution||1 x 15ml|
|VAP-1 Standard lyophilized||2 vials|
Our Abpromise guarantee covers the use of ab119564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119564 has not yet been referenced specifically in any publications.