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Abcam’s anti-Varicella-Zoster virus (VZV) IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Varicella-Zoster virus (VZV) in Human serum and plasma.
A 96-well plate has been precoated with Varicella-Zoster virus (VZV) antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Varicella-Zoster virus (VZV)-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Varicella-Zoster virus (VZV) IgA sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover foil||1 unit|
|IgA Sample Diluent||Colored yellow; White cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
|Varicella-Zoster virus (IgA) Coated Microplate (12 x 8 wells)||12 strips of 8 wells||1 unit|
|Varicella-Zoster virus anti-IgA HRP Conjugate||Colored violet; Black cap||1 x 20ml|
|Varicella-Zoster virus IgA Cut-off Control||Colored yellow; Green cap||1 x 3ml|
|Varicella-Zoster virus IgA Negative Control||Colored yellow; Blue cap||1 x 2ml|
|Varicella-Zoster virus IgA Positive Control||Colored yellow; Red cap||1 x 2ml|
Our Abpromise guarantee covers the use of ab108781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab108781 has not yet been referenced specifically in any publications.