Human VCAM-1 ELISA Set (without plates) (ab47355)

Overview

  • Product nameHuman VCAM-1 ELISA Set (without plates)
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.6 ng/ml
  • Range
    1.56 ng/ml - 50 ng/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s VCAM-1 Human in vitro ELISA Set is designed for the quantitative measurement of VCAM-1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    This ELISA Set is intended to measure human VCAM-1 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

    The VCAM-1 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    Recognizes both natural and recombinant human VCAM1

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformReagents

Properties

  • RelevanceVCAM1 is important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the integrins alpha4 beta1 (beta 1 integrin VLA4) and alpha4 beta7 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. VCAM1 is also expressed by several non endothelial cell types including some macrophages, follicular dendritic cells and bone marrow, stromal cells.
  • Cellular localizationCell Membrane; single-pass type I membrane protein
  • Alternative names
    • CD106
    • CD106 antigen
    • INCAM 100
    • L1CAM
    • Vascular cell adhesion molecule 1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab47355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human VCAM-1 ELISA Set (without plates) images

  • VCAM-1 Standard curve ranging from 1.56 to 50 ng/mL

References for Human VCAM-1 ELISA Set (without plates) (ab47355)

ab47355 has not yet been referenced specifically in any publications.

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Please find below additional info required for ab47355 and ab47361


1) PBS is prepared as followed : 0.137mM NaCl; 1.47 KH2PO4; 8.1mM Na2PO4, 2H2O

2) Samples should be diluted : 1/100 to 1/2...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"