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Abcam’s Human VCAM1 (CD106) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of VCAM1 in cell culture supernatants, buffered solutions, serum, plasma, and other body fluids.
A monoclonal antibody specific for VCAM1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known VCAM1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for VCAM1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of VCAM1 present in the samples.
This kit will recognize both endogenous and recombinant Human VCAM1.
|Components||Identifier||2 x 96 tests||1 x 96 tests|
|10X Standard Diluent Buffer||Black||1 x 25ml||1 x 25ml|
|200X Wash Buffer||White||2 x 10ml||1 x 10ml|
|Biotinylated Antibody Diluent||Red||1 x 13ml||1 x 7.5ml|
|Biotinylated anti-VCAM1||Red||2 x 400µl||1 x 400µl|
|Chromogen TMB Substrate Solution||1 x 24ml||1 x 11ml|
|Control||Silver||4 vials||2 vials|
|HRP Diluent||Red||1 x 23ml||1 x 23ml|
|Stop Reagent||Black||2 x 11ml||1 x 11ml|
|Streptavidin-HRP||4 x 5µl||2 x 5µl|
|VCAM1 Microplate (12 x 8 well strips)||2 units||1 unit|
|VCAM1 Standard (lyophilized)||Yellow||4 vials||2 vials|
Our Abpromise guarantee covers the use of ab46118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab46118 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"