|Sample type||Average %||Range|
|Cell culture extracts||96.79||90% - 107%|
|Tissue Extracts||95.58||89% - 106%|
Abcam’s VEGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF in cell lysates and tissue lysates.
This assay employs an antibody specific for Human VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|2X Cell Lysate Buffer||1 x 5ml|
|300X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent||1 x 15ml|
|5X Sample Diluent Buffer||1 x 10ml|
|Biotinylated anti-Human VEGF||2 vials|
|Recombinant Human VEGF Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
|VEGF Microplate (12 x 8 wells)||1 unit|
Our Abpromise guarantee covers the use of ab100663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Hu VEGF measured in cell lysates showing quantity (pg) per million cells tested
Hu VEGF in various fluids showing quantity (pg) per mL of tested sample
Standard curve : mean of duplicates (+/-SD) with background readings subtracted