For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
|Sample type||Average %||Range|
|Cell culture supernatant||47% - 79%|
|Serum||46% - 67%|
Save time with a 90-minute ELISA protocol. Try Human VEGF R1 ELISA Kit (FLT1) (ab195210) from our SimpleStep ELISA® range.
Abcam’s VEGF R1 (FLT1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF R1 (FLT1) in cell culture supernatant, serum and plasma (EDTA, citrate).
VEGF R1 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated VEGF R1 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of VEGF R1 captured on the plate.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||4 units|
|Biotin-Conjugate anti-Human VEGF R1 polyclonal antibody||1 x 100µl|
|Conjugate Diluent||1 x 20ml|
|Microplate coated with monoclonal antibody to Human VEGF R1 (12 x 8 wells)||1 unit|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 150µl|
|TMB Substrate Solution||1 x 15ml|
|VEGF R1 Standard lyophilized||2 vials|
Our Abpromise guarantee covers the use of ab119567 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|