Human VEGF R1 ELISA Kit (FLT1) (ab119613)
Key features and details
- Sensitivity: 30 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human VEGF R1 ELISA Kit (FLT1)
See all VEGF Receptor 1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 237pg/ml 9.71 = 4.1% 2 16 1369pg/ml 106.78 = 7.8% 3 16 4420pg/ml 203.32 = 4.6% Inter-assay Sample n Mean SD CV% 1 24 240pg/ml 11.28 = 4.7% 2 24 1352pg/ml 131.14 = 9.7% 3 24 4316pg/ml 202.85 = 4.7% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 30 pg/ml -
Range
156 pg/ml - 10000 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human soluble VEGF R1 (FLT1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human soluble VEGF R1 (FLT1) in cell culture supernatants, serum and plasma (EDTA).
A VEGF R1 (FLT1) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for VEGF R1 (FLT1) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution The density of yellow coloration is directly proportional to the Human VEGF R1 (FLT1) amount of sample captured in plate.
Get higher sensitivity in only 90 minutes with Human VEGF R1 ELISA Kit (FLT1) (ab195210) from our SimpleStep ELISA® range.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human VEGF Receptor 1 antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human VEGF Receptor 1 antibody 1 x 100µl Lyophilized recombinant Human VEGF Receptor 1 standard 2 x 10ng Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis. -
Tissue specificity
Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform sFlt1 is strongly expressed in placenta. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- EC 2.7.10.1
- FLT
- FLT 1
see all -
Database links
- Entrez Gene: 2321 Human
- Omim: 165070 Human
- SwissProt: P17948 Human
- Unigene: 594454 Human
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab119613 has been referenced in 3 publications.
- Buell-Acosta JD et al. Maternal Fibroblast Growth Factor 21 Levels Decrease during Early Pregnancy in Normotensive Pregnant Women but Are Higher in Preeclamptic Women-A Longitudinal Study. Cells 11:N/A (2022). PubMed: 35883694
- Garcés MF et al. Maternal Serum Meteorin Levels and the Risk of Preeclampsia. PLoS One 10:e0131013 (2015). PubMed: 26121675
- Kinoshita Y et al. Mead acid inhibits the growth of KPL-1 human breast cancer cells in vitro and in vivo. Oncol Rep 32:1385-94 (2014). PubMed: 25109488