Overview

  • Product nameHuman VEGF Receptor 2 ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 70 pg/ml
  • Range
    34.3 pg/ml - 25000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 96.23 86% - 104%
    Serum 96.46 85% - 105%
    Plasma 94.78 84% - 103%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s VEGF Receptor 2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF Receptor 2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human VEGF Receptor 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF Receptor 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF Receptor 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF Receptor 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceVEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Cellular localizationCell Membrane, Cytoplasmic, Nuclear and Secreted
  • Alternative names
    • CD309
    • Fetal liver kinase 1
    • FLK1
    • KDR
    • Kinase insert domain receptor
    • Kinase insert domain receptor (a type III receptor tyrosine kinase)
    • Protein tyrosine kinase receptor Flk 1
    • Tyrosine kinase growth factor receptor
    • Vascular endothelial growth factor receptor 2
    • VEGFR
    • VEGFR 2
    • VEGFR2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human VEGF Receptor 2 ELISA Kit images

  • Representative standard curve using ab100665

  • Representative standard curve using ab100665

Protocols

References for Human VEGF Receptor 2 ELISA Kit (ab100665)

ab100665 has not yet been referenced specifically in any publications.

Product Wall

The lab does test this kit with cells cultured in serum-free media, so we aren't sure how serum in the media will directly affect the results. We suggest testing a few different culturing conditions with varying serum percent levels to find the level t...

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Our tests show that there is no cross reactivity with mouse VEGF R2 and the Human VEGF R2 ELISA.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more ad...

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Thank you for contacting Abcam regarding this.

Unfortunately because this information is considered proprietary, I am not able to share the exact contents of the standard/sample diluent other than to tell you it is an animal serum with 0.09%...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"