Human Vitronectin full length protein (ab92639)

Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids0 to 0
  • ConjugationBiotin

Specifications

Our Abpromise guarantee covers the use of ab92639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity> 95 % by SDS-PAGE.
    ab92639 is prepared from fresh Human plasma using non-denaturing chromatography, then biotin labeled at primary amines. Purity is > 98% pure by SDS-PAGE.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 0.1M Sodium chloride, 0.05M Sodium phosphate, pH 7.4

General info

  • Alternative names
    • Complement S Protein
    • Epibolin
    • S Protein
    • S-protein
    • Serum Spreading Factor
    • Serum-spreading factor
    • Somatomedin B
    • Somatomedin-B
    • V75
    • Vitronectin V10 subunit
    • Vitronectin V65 subunit
    • VN
    • VNT
    • VTN
    • VTNC_HUMAN
    see all
  • FunctionVitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
    Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
  • Tissue specificityPlasma.
  • Sequence similaritiesContains 4 hemopexin-like domains.
    Contains 1 SMB (somatomedin-B) domain.
  • DomainThe SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
  • Post-translational
    modifications
    Sulfated on 2 tyrosine residues.
    N- and O-glycosylated.
    Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt

Human Vitronectin full length protein images

  • 10% SDS-PAGE
    Lane 1: Vitronectin (2 ug) Reduced
    Lane 2: Vitronectin (2 ug) Non-Reduced
    Lane 3: Prestained Standard
  • All lanes : anti-Vitronectin Avidin-AP at 1/3000 dilution

    Lane 1 : ab92639 (Reduced)
    Lane 2 : ab92639 (Non-reduced)

    Lysates/proteins at 2 µg per lane.


    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

References for Human Vitronectin full length protein (ab92639)

ab92639 has not yet been referenced specifically in any publications.

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I apologize but the information on our datasheet is incorrect. The labeling ratio actually varie...

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