Abcam’s Von Willebrand Factor (VWF) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Von Willebrand Factor in plasma, serum, and cell culture supernatants.
A Von Willebrand Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Von Willebrand Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Von Willebrand Factor captured in plate.
Get higher sensitivity in only 90 minutes with Human Von Willebrand Factor ELISA Kit (ab189571) from our SimpleStep ELISA® range.
|Components||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent N Concentrate||1 x 30ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|50X Biotinylated Human Von Williebrand Factor Antibody||1 x 120µl|
|Chromogen Substrate||1 x 8ml|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
|Von Williebrand Factor Microplate (12 x 8 well strips)||1 unit|
|Von Williebrand Factor Standard||1 vial|
Our Abpromise guarantee covers the use of ab108918 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent dilution.|
VWF measured in biological fluids with background signal subtracted (duplicates +/- SD). There is limited cross-reactivity in mouse (< 2%) and rat samples (< 15%).
Representative Standard Curve using ab108918