For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Abcam’s Von Willebrand Factor (VWF) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Von Willebrand Factor levels in cell culture supernatants, plasma and serum.
A Von Willebrand Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Von Willebrand Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Von Willebrand Factor captured in plate.
Get higher sensitivity in only 90 minutes with Human Von Willebrand Factor ELISA Kit (ab189571) from our SimpleStep ELISA® range.
|Components||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent N Concentrate||1 x 30ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|50X Biotinylated Human Von Williebrand Factor Antibody||1 x 120µl|
|Chromogen Substrate||1 x 8ml|
|Negative control (Depleted Human Plasma)||1 vial|
|Positive control (Reference Plasma Control)||1 vial|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
|Von Williebrand Factor Microplate (12 x 8 well strips)||1 x 96 tests|
|Von Williebrand Factor Standard||1 vial|
Our Abpromise guarantee covers the use of ab168548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab168548 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"