Human Von Willebrand Factor ELISA Kit (with plasma controls) (ab168548)

Overview

  • Product name
    Human Von Willebrand Factor ELISA Kit (with plasma controls)
    See all Von Willebrand Factor kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    > 2.5 mU/ml
  • Range
    2.5 mU/ml - 80 mU/ml
  • Recovery

    98.5 %

  • Assay time
    5h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Von Willebrand Factor (VWF) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Von Willebrand Factor levels in cell culture supernatants, plasma and serum.


    A Von Willebrand Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Von Willebrand Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Von Willebrand Factor captured in plate.


    Get higher sensitivity in only 90 minutes with Human Von Willebrand Factor ELISA Kit (ab189571) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Von Williebrand Factor Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Negative control (Depleted Human Plasma) 1 vial
    Positive control (Reference Plasma Control) 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
    Von Williebrand Factor Microplate (12 x 8 well strips) 1 x 96 tests
    Von Williebrand Factor Standard 1 vial
  • Research areas
  • Function
    Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
  • Tissue specificity
    Plasma.
  • Involvement in disease
    Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
  • Sequence similarities
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 4 TIL (trypsin inhibitory-like) domains.
    Contains 3 VWFA domains.
    Contains 3 VWFC domains.
    Contains 4 VWFD domains.
  • Domain
    The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
  • Post-translational
    modifications
    All cysteine residues are involved in intrachain or interchain disulfide bonds.
    N- and O-glycosylated.
  • Cellular localization
    Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
  • Information by UniProt
  • Alternative names
    • Coagulation factor VIII
    • Coagulation factor VIII VWF
    • F8VWF
    • Factor VIII related antigen
    • von Willebrand antigen 2
    • von Willebrand antigen II
    • Von Willebrand disease
    • VWD
    • vWF
    • VWF_HUMAN
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab168548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab168548

Protocols

References

ab168548 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

I can confirm that the ELISA kits ab108918 and ab168548 are identical apart from ab168548 has a positive and negative plasma control included. The antibodies used in both of these kits are indeed the same.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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