Overview

  • Product name
    Human ZAP70 ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Jurkat 5 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Jurkat 3 6.5%
  • Sample type
    Cell culture extracts, Adherent cells, Suspension cells, Cell Lysate, Tissue Homogenate
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 500 pg/ml
  • Range
    1.6 ng/ml - 100 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture media 90 89% - 91%
    Fetal Bovine Serum 57 48% - 61%
    Bovine Serum Albumin 106 103% - 111%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
    Predicted to work with: RatDoes not react with: Mouse
  • Product overview

    Abcam’s ZAP70 Human in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of catalase protein in Human cell and tissue extracts.

    The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    ZAP70 is a tyrosine kinase that plays an essential role in the regulation of the adaptive immune response. ZAP70 regulates motility, adhesion and cytokine expression of mature T-cells, as well as in thymocyte development. ZAP70 regulates T-cell activation by modulating T-Cell Receptor expression at the cell surface.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab173192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Example ZAP70 standard curve.  Background-subtracted data values (mean +/- SD) are graphed. Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

  • Titration of Jurkat extract within the working range of the Zap70 assay. Background subtracted data from duplicate measurements are plotted.

  • Interpolated values of ZAP70 are graphed for the indicated cells lines based on a extract load of 12.5 µg/mL. The ZAP70 detector antibody was used to analyze the same extracts by western blot (20 µg loaded/lane). The actin blot is included to show the relative loads of each lysate.

Protocols

References

ab173192 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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