Overview

  • Product name
  • Description
    Rabbit polyclonal to Huntingtin
  • Host species
    Rabbit
  • Specificity
    ab155942 recognizes the 552 cleaved fragment without detecting the full-length form.
  • Tested applications
    Suitable for: IHC-Fr, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Huntingtin aa 544-552 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue).
    Sequence:

    SDPAMDLND


    Database link: P42858

  • Positive control
    • Huntingtin protein and protein fragments; 293T cells; Mouse cortex tissue; Endogenous Huntingtin lysates, with or without caspase activity.
  • General notes

    Human HTT caspase cleavage sites generate fragment-specific forms of the protein. Caspase-3/7 has been shown to generate cleavage sites at animo acids 513 and 552. Caspase-2 cleaves at amino acid 552 and caspase-6 at amino acid 586.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Human HTT caspase cleavage sites generate fragment-specific forms of the protein. Caspase-3/7 has been shown to generate cleavage sites at animo acids 513 and 552. Caspase-2 cleaves at amino acid 552 and caspase-6 at amino acid 586.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50 - 1/200.
WB 1/500. Predicted molecular weight: 348 kDa.
ELISA 1/20 - 1/100.
ICC/IF 1/50 - 1/200.

Target

  • Function
    May play a role in microtubule-mediated transport or vesicle function.
  • Tissue specificity
    Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
  • Involvement in disease
    Defects in HTT are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum).
  • Sequence similarities
    Belongs to the huntingtin family.
    Contains 10 HEAT repeats.
  • Domain
    The N-terminal Gln-rich and Pro-rich domain has great conformational flexibility and is likely to exist in a fluctuating equilibrium of alpha-helical, random coil, and extended conformations.
  • Post-translational
    modifications
    Cleaved by apopain downstream of the polyglutamine stretch. The resulting N-terminal fragment is cytotoxic and provokes apoptosis.
    Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.
  • Cellular localization
    Cytoplasm. Nucleus. The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington's disease neurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI256365 antibody
    • C430023I11Rik antibody
    • HD antibody
    • HD protein antibody
    • HD_HUMAN antibody
    • HDH antibody
    • HTT antibody
    • Huntingtin antibody
    • HUNTINGTON CHOREA antibody
    • Huntington disease protein antibody
    • Huntington's disease protein homolog antibody
    • IT 15 antibody
    • IT15 antibody
    • OTTMUSP00000026909 antibody
    • ZHD antibody
    see all

Images

  • All lanes : Anti-Huntingtin antibody (ab155942) at 1/500 dilution

    Lane 1 : -
    Lane 2 : +C2
    Lane 3 : +C3
    Lane 4 : +C6
    Lane 5 : +C7
    Lane 6 : Huntingtin23Q(1-513)
    Lane 7 : Huntingtin23Q(1-536)
    Lane 8 : Huntingtin23Q(1-552)
    Lane 9 : Huntingtin23Q(1-586)

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 348 kDa
    Additional bands at: 62 kDa (possible cleavage fragment)



    Lanes 1-5 are 293Tcells expressing the full length Htt protein and treated with the various caspases. Lanes 6-9 are 293T overexpressing the different Htt fragments to demonstrate the antibody’s specificity to that neo-epitope. Probed with N-terminal pan-Huntingtin antibody or (bottom panel) neoepitope specific rabbit polyclonal antibody.
  • Sandwich Elisa analysis of 293T cells (overexpressing Huntingtin neo epitope 552), labeling Huntingtin protein (0, 0.5,1,1.5 and 2µg) with ab155942 at 1/20 dilution.
  • Immunofluorescent analysis of 293T cells (transfected with Huntingtin23Q and Huntingtin148Q stop constructs ending in amino acid 552), labeling Huntingtin ab155942 at 1/50 dilution.
  • Immunohistochemistry analysis of Mouse cortex tissue samples, labeling Huntingtin with ab155942 at 1/50 dilution (green). Blue: Tissue counterstained with nuclei stain.

References

ab155942 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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